1. 个人简介:
王文方 博士,讲师
教育经历:
2015-2020,博士,上海交通大学
2011-2014,硕士,中国科大红鹰dhy9999大学
2007-2011,学士,郑州大学
工作经历:
2022-至今,上海师范大学 讲师
2020-2022,上海师范大学 师资博士后
2. 主要研究方向:
[1] 微生物天然产物合成生物学研究。
[2] 基因组编辑技术研究。
3. 代表性科研项目:
[1] 刺糖多孢菌中次级代谢启动子驱动的CRISPRi自主动态调控系统的创建及应用,中国博士后科学基金,2022-2023。
[2] 与合成生物学研究契合的课程思政研究,上海高校青年教师培养资助计划重点资助,2023-2025。
[3]新型CRISPRa/i技术驱动的多杀菌素生物合成代谢网络优化,微生物代谢国家重点实验室开放课题,2023-2025。
[4]基于新型小Cas12f蛋白的放线菌基因组编辑技术的开发及应用,系统生医学教育部重点实验室开放课题,2023-2025。
4. 代表性论文及著作:
[1] Hui-Min Hua, Jia-Feng Xu, Xue-Shuang Huang*, Andrei A. Zimin, Wen-Fang Wang*, and Yin-Hua Lu*, Low-toxicity and high-efficiency Streptomyces genome editing tool based on the miniature type V–F CRISPR/Cas nuclease AsCas12f1, Journal of Agricultural and Food Chemistry, 2024, 72, 5358-5367.
[2] Jia-Xiang Ma, Wen-Yan He, Hui-Min Hua, Qian Zhu, Guo-Song Zheng, Andrei A. Zimin, Wen-Fang Wang*, and Yin-Hua Lu*, Development of a CRISPR/Cas9D10A nickase (nCas9)-mediated genome editing tool in Streptomyces, ACS synthetic biology, 2023, 12(10):3114-3124.
[3] Wenyan He#; Wenfang Wang#; Jiaxiang Ma; Guosong Zheng; Andrei A. Zimin; Weihong Jiang; Jinzhong Tian; Yinhua Lu; Crossregulation of rapamycin and elaiophylin biosynthesis by RapH in Streptomyces rapamycinicus, Applied Microbiology and Biotechnology, 2022, 106(5-6):2147-2159.
[4]Wenfang Wang, Guosong Zheng, Yinhua Lu*; Recent advances in strategies for the cloning of natural product biosynthetic gene clusters, Frontiers in Bioengineering and Biotechnology, 2021, 9: 692797.
[5] Guosong Zheng, Panpan Liu, Wenyan He, Hengnuo Tao, Zhen Yang, Chuanwen Sun, Wenfang Wang*, Yinhua Lu*, Weihong Jiang; Identification of the cognate response regulator of the orphan histidine kinase OhkA involved in both secondary metabolism and morphological differentiation in Streptomyces coelicolor, Applied Microbiology and Biotechnology, 2021, 105(14): 5905-5914.
[6]Wenfang Wang*; Han Xiao; Jianjiang Zhong*; Biosynthesis of a novel ganoderic acid by expressing CYP genes from Ganoderma lucidum in Saccharomyces cerevisiae, Applied Microbiology and Biotechnology, 2021, 106(2): 523-534.
[7] 王文方,钟建江,《合成生物学驱动的智能生物制造研究进展》,生命科学,2019,31(4): 413-422。
[8]Wang Wenfang#, Xiao Han#, Zhong Jianjiang*. Biosynthesis of a ganoderic acid in Saccharomyces cerevisiae by expressing a cytochrome P450 gene from Ganoderma lucidum, Biotechnology and Bioengineering, 2018, 115(7):1842-1854.
5. 代表性授权专利:
[1] 肖晗、王文方、钟建江,通过合成生物学手段异源生物合成灵芝酸的方法, 2017-5-13,授权号CN108929884。
更新时间:2024年5月